Rat Secondary Antibodies
Biotium HA Tag(16.43), Biotin conjugate, 0.1mg/mL
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium CD86(C86/1146), CF405S conjugate, 0.1mg/mL
Recognizes a protein of 70 kDa, which is identified as CD86. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4, a cancer immunotherapy target. It is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin€™s disease.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note:
Rockland Immunochemicals Mouse TrueBlot: Anti-Mouse Ig Biotin - 13-8817-82
Mouse IgG TrueBlot ULTRA is a unique Anti-mouse IgG monoclonal secondary antibody. Mouse IgG TrueBlot ULTRA enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication‐quality IP/Western blotting with Mouse IgG TrueBlot ULTRA, simply substitute the conventional Anti‐Mouse IgG blotting reagent with Mouse IgG TrueBlot ULTRA and follow the prescribed protocol for sample preparation and immunoblotting. The Biotin Mouse TrueBlot ULTRA is the biotinylated format of Mouse IgG TrueBlot ULTRA. It can be used as a secondary antibody, followed by the use of avidin-peroxidase conjugated (A003-03).
